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. 2024 Jul 2;27(7):1260–1273. doi: 10.1038/s41593-024-01677-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — (a-d) Representative micrographs of astrocytes immunostained for the indicated proteins at day 2 and 4 after induction of the inducible constructs expressing GFP, Ngn2 or PmutNgn2. Scale bar 20 µm. In (c), the filled arrow heads mark the iNs, the empty arrow heads mark example cells lacking neuronal markers. In (d), the filled arrow heads mark mature iNs (NeuN+Dcx-), the empty arrow marks immature iNs (NeuN+Dcx + ). (e) Histograms depicting cell quantifications indicated on the y-axis over time indicated on the x-axis. Data are plotted as mean ± standard deviation (sd) where the dots represent individual biological replicates (n = 3).