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. 2024 Jul 11;15:5830. doi: 10.1038/s41467-024-49297-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic diagram showing the procedure for reconstitution of TMEM87A into liposome (8:2, POPC:POPG) with dehydration/rehydration method for single-channel recording. b Representative spontaneous single-channel currents from the reconstituted proteoliposome of TMEM87A under voltage steps (from +90 to −150 mV, 30 mV step) from the same patch condition. c Voltage-dependent channel-opening probability (Po) of TMEM87A at each holding potential (n = 3). d I-V relationship of TMEM87A single-channel unitary current activities (n = 3). Data were fitted with a polynomial. e The unitary current × open probability-voltage relationship of TMEM87A (n = 3). f Amplitude histogram of TMEM87A single-channel unitary current activities with open (O) and closed (C) states at +90 mV (orange) and −150 mV (purple) from (b). Distribution data are fitted with a sum of two Gaussians at each holding potential. Data were presented as the mean ± SEM. The n numbers are from three independent proteoliposome experiments. Source data are provided as a Source Data file.