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. 2024 Jul 11;15:5830. doi: 10.1038/s41467-024-49297-8

Fig. 8. GolpHCat contributes to hippocampal spatial and contextual memories.

Fig. 8

a Schematic diagram of fEPSP recording in the Schaffer-collaterals pathway. b Input-output curve for fEPSP slope obtained with increasing stimulus intensities in WT and GolpHCat KO mice. c Paired-pulse ratios (PPR) obtained with increasing interpulse intervals. Inset: representative fEPSP traces. Scale bar: 1 mV and 500 ms. d HFS (1 s at 100 Hz)-induced LTP. Inset: representative fEPSP traces from before and after HFS-induced LTP. Scale bar: 0.5 mV and 10 ms. e Slope of fEPSP over the last 5 min. n = 10 cells from three mice for WT and n = 14 cells from three mice for GolpHCat KO in (b–e). f Schematic diagram of the NPR and contextual fear task. g Total object exploration time during the test phase of the NPR task. h Discrimination index during the test phase of the NPR task. i Percentage of freezing during the acquisition phase of contextual fear task (left) and retrieval phase during the 5 min (right). The orange rectangular indicates the time point of shock. n = 9 mice for WT and n = 9 mice for GolpHCat KO in (g–i). j Illustration of virus injection for Scrambled (n = 9) and astrocyte-specific GolpHCat KD (Astrocytic KD; n = 9) in the stratum radiatum of the hippocampal CA1 region. k Total object exploration time during the test phase of the NPR task. l Discrimination index during the test phase of the NPR task. m Percentage of freezing during the acquisition phase of contextual fear task (left). Percentage of freezing during the retrieval phase during the 5 min (right). The pink rectangular indicates the time point of shock. n = 9 mice for Scrambled and n = 9 mice for Astrocytic KD in (k–m). n Illustration of virus injection for Scrambled and neuron-specific GolpHCat KD (Neuronal KD) in the pyramidal layer of the hippocampal CA1 region. o Total object exploration time during the test phase of the NPR task. p Discrimination index during the test phase of the NPR task. q Percentage of freezing during the acquisition phase of contextual fear task (left). Percentage of freezing during the retrieval phase during the 5 min (right). The purple rectangular indicates the time point of shock. n = 8 mice for Scrambled and n = 10 mice for Neuronal KD in (o–q). Data were presented as the mean ± SEM. Statistical analyses were performed using two-way ANOVA followed by Šídák’s multiple comparisons test in b (F(6,32) = 0.8259), c (F(6,132) = 1.021), i acquisition, (F(10,160) = 0.7738), m acquisition (F(10,160) = 0.2676), q acquisition (F(10,160) = 1.002); two-tailed unpaired t-test in e (t = 2.426, df = 22), i retrieval (t = 2.367, df = 16), l (t = 2.786, df = 16), m retrieval (t = 2.502, df = 16), and p (t = 2.523, df = 16); two-tailed paired t-test in g (WT (t = 4.361, df = 8), KO (t = 2.275, df = 8), k (Scrambled (t = 2.969, df = 8), Astrocytic KD (t = 0.3143, df = 8), o (Scrambled (t = 2.600, df = 7), Neuronal KD (t = 0.1794, df = 9); two-tailed Mann–Whitney test in (h) (U = 0), and q retrieval (U = 16). Source data and exact p values are provided as a Source Data file.