FIG. 1.

Distribution of adenovirus during acute respiratory tract infection. Infectious, fluorescently labeled adenovirus (Ad-Cy3) or HBSS, as a sham control, was administered by intratracheal instillation into the lungs of mice. Mice were then sacrificed after either 1 min (left panels) or 10 min (right panels), and the lungs were removed and processed for tissue sections or the mice were subjected to BAL, followed by recovery and cytospin preparation of cells as described in Materials and Methods. Shown are fluorescence and corresponding phase photomicrographs for tissue sections (top and middle panels, ×114) and fluorescence and bright-field photomicrographs for BAL cells (bottom panels, ×232). For BAL cells, separate slides were prepared for fluorescence and bright-field photomicroscopy because Diff-Quick staining partially quenched Cy3 fluorescence.