FIG. 4.

Localization of TNF-α mRNA expression by in situ hybridization in lung tissues 6 h after adenovirus respiratory tract infection. Mice were exposed to adenovirus or HBSS and then sacrificed and prepared for in situ hybridization as described in the legend to Fig. 3. Tissues were hybridized with TNF-α-specific ³⁵S-labeled antisense and sense cRNA probes. Dark-field (above) and bright-field (below) views of the hybridizations of lung tissues from adenovirus-infected or sham control (HBSS)-exposed mice with antisense or sense probes (indicated) are shown. The bronchial epithelium (br) and vascular endothelium (v) are indicated. The sense probe did not show specific hybridization in HBSS-exposed mouse lung (not shown but similar to sense probe [middle panel] in adenovirus-infected mice). (Left and middle panels, ×143; right panels, ×286).