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Analysis of adenovirus-mediated transgene delivery and expression. AMφ, RAW264.7 macrophages, or A549 cells, as an epithelial-like cell positive control, were infected with Av1GFP, an adenovirus vector similar in structure to Av1nBg except expressing a mammalianized GFP encoding transgene. After 48 h, cells were evaluated for GFP expression by quantifying fluorescence using flow cytometry (gray profiles). Uninfected control cell samples were also evaluated (black profiles).
