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. 2024 Jun 7;25(7):3040–3063. doi: 10.1038/s44319-024-00170-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A, B) Immunofluorescence staining of SDCCAG3 (A) or IFT20 (B), both in yellow, and acetylated α-tubulin (AcTub, magenta) in kidney sections from wild-type and Pax3Cre-Dlg1^F/F mice. (C) Quantification of relative MFI of SDCCAG3 and IFT20 in cilia of wild-type (n = 3) and Pax3Cre-Dlg1^F/F (n = 4) mice, respectively. The levels from control mice were set to 1, and the ciliary levels from mutant mice were compared to that (i.e., relative fluorescence intensity). Data shown are the average values from each mouse. The vertical segments in box plots show the first quartile, median, and third quartile. The whiskers on both ends represent the maximum and minimum values for each dataset analyzed. Statistical analysis was performed two-tail unpaired t-test. *P < 0.05, **P < 0.01. Source data are available online for this figure.