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. 2024 May 23;31(7):897–909. doi: 10.1038/s41418-024-01313-6

Fig. 3. In situ detection of pMLKL-S345 is applicable to immunofluorescence staining.

Fig. 3

A, B Representative images of skin sections from mice with the indicated genotypes immunostained with Alexa fluor 594 streptavidin (red) to detect pMLKL-S345 and with DAPI (blue) to detect nuclei (n = 5 in each group). Dashed squares indicate 4 representative pMLKL-S345-positive cells per image. Scale bars: 100 µm. B Representative images (single slice 0.4 μm) of pMLKL-S345 positive cells (n = 4 cells per mouse in a total of n = 5 mice). Scale bar: 5 µm.