Fig. 4. SARS-CoV infection induces apoptotic and necroptotic cell death.

A Representative bright field images of lung sections of wt mice infected intranasally with 10⁶ TCID50/ml SARS-CoV MA15 virus at 3 days post-infection (dpi) immunostained with pMLKL-S345 (n = 3 for mock-infected and MA15-infected mice). Scale bars: 100 µm (representative field) and 50 µm (magnified selected area). B Graph showing quantification of pMLKL-S345 immunostaining obtained via QuPath after slides were digitalised in a digital slide scanner as described in the Supplementary Methods section. Total lung sections were analysed and total numbers of cells were obtained to calculate the percentage of positive cells over the total amount of cells detected. Data are presented as mean + SEM and each dot represents one mouse. P values were calculated via one-way Anova, Tukey’s multiple comparisons test. *P ≤ 0.05.