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. 2024 Jul 12;14:16086. doi: 10.1038/s41598-024-66970-6

Figure 4.

Figure 4

Screening for the specificity of the nanobody using 5XFAD mouse brain slides. (A) Ex-vivo evaluation of SAβO-binding specificity of the nanobody clones using consecutive brain slides obtained from a WT (6-month-old) and a 5XFAD mouse (11-month-old). (B) Ranking of nanobody clones after pixel quantification of 5XFAD brain slides. The threshold of each image was adjusted until the background disappeared using ImageJ. This process was repeated 3 times, and the data were presented as a mean ± SD.