TABLE 2.

PBMC proliferation in response to E1, E2, E6, and E7 stimulation in vitro in vaccinated and control rabbits

Rabbita	PBMC proliferation (103 cpm; mean ± SEM)b					SIc
	E1	E2	E6	E7	No antigen	E1	E2	E6	E7
Vaccinated
1	4.442 ± 0.382	1.083 ± 0.162	0.382 ± 0.025	0.699 ± 0.046	0.346 ± 0.039	12.8	3.0	1.1	2.0
2	0.613 ± 0.064	0.584 ± 0.068	0.489 ± 0.106	0.778 ± 0.100	0.328 ± 0.058	1.9	1.8	1.5	2.4
3	3.223 ± 0.437	2.179 ± 0.232	0.681 ± 0.081	1.809 ± 0.236	0.457 ± 0.050	7.1	4.8	1.5	4.0
4d	NDe	ND	ND	ND	ND	ND	ND	ND	ND
5	16.50 ± 2.923	4.124 ± 1.658	0.327 ± 0.061	0.523 ± 0.164	0.200 ± 0.067	82.5	20.6	1.6	2.6
6	0.814 ± 0.049	0.635 ± 0.045	0.530 ± 0.068	1.540 ± 0.367	0.421 ± 0.016	1.9	1.5	1.3	3.7
7	0.368 ± 0.016	0.430 ± 0.054	0.146 ± 0.016	0.253 ± 0.031	0.142 ± 0.007	2.6	3.0	1.0	1.8
8	0.696 ± 0.120	0.264 ± 0.013	0.222 ± 0.016	0.312 ± 0.060	0.204 ± 0.020	3.4	1.3	1.1	1.5
Control
9	1.554 ± 0.222	0.675 ± 0.142	0.322 ± 0.015	0.417 ± 0.014	0.246 ± 0.019	6.3	2.7	1.3	1.7
10	0.628 ± 0.161	0.382 ± 0.094	0.265 ± 0.080	0.393 ± 0.019	0.199 ± 0.008	3.1	1.9	1.3	2.0
11	0.324 ± 0.065	0.265 ± 0.022	0.324 ± 0.072	0.836 ± 0.219	0.310 ± 0.054	1.0	0.9	1.0	2.6
12d	ND	ND	ND	ND	ND	ND	ND	ND	ND
13	1.616 ± 0.301	0.657 ± 0.047	0.406 ± 0.010	0.504 ± 0.080	0.428 ± 0.027	3.8	1.5	0.9	1.2
14	0.624 ± 0.068	0.665 ± 0.052	0.359 ± 0.068	0.408 ± 0.036	0.312 ± 0.023	2.0	2.1	1.6	1.3
15	0.478 ± 0.065	0.730 ± 0.094	0.381 ± 0.068	0.255 ± 0.039	0.252 ± 0.051	1.9	2.9	1.5	1.0
16	0.261 ± 0.022	0.309 ± 0.043	0.208 ± 0.012	0.228 ± 0.017	0.165 ± 0.016	1.6	1.9	1.3	1.4

^aRabbits were vaccinated with E1, E2, E6, and E7 genes; control rabbits received vector alone. 

^bValues for three wells. All values were determined from assays done on day 4. 

^cRatio of counts per minute in response to specific antigen to counts per minute in response to no antigen. A SI greater than 2 was considered positive and is shown in bold type. 

^dInsufficient PBMC for assays. 

^eND, not done.