Figure 5.

Survival rate of V. anguillarum and V. ordalii against total protein extracts from C. gilberti epidermal mucus after 6 days of fish immunostimulation, previously exposed to normoxic and chronic hypoxic conditions. Data are expressed as optical density at 570 nm and presented as mean ± SD (n = 3). Index: C+: blank; G: gentamicin (activity control); C−: bacterial growth control; N: normoxia group; H: hypoxia group; PBS: non-stimulated group; LPS: fish intraperitoneally inoculated with LPS of V. anguillarum. Different letters indicate statistically significant differences (p ≤ 0.05).