Table 2.
Lysozyme activity (U mL−1) in Chilean meagre epidermal mucus and equivalent amount of chicken egg lysozyme (µg) per 100 µg of mucus total proteins. Results obtained from samples collected during acute and chronic hypoxia experiments.
| Experiment | Time Point | Treatment | Lysozyme Activity (U mL−1) | Equivalent Amount of Egg Lysozyme (µg per 100 µg of Sample) |
|---|---|---|---|---|
| Acute hypoxia | After hypoxia (3 h) | Normoxia | ND | - |
| Hypoxia | 4.00 ± 5.66 | 0.09 ± 0.13 | ||
| After immunostimulation |
N-PBS | 10.00 ± 2.83 | 0.19 ± 0.01 | |
| N-VA | ND | - | ||
| H-PBS | ND | - | ||
| H-VA | ND | - | ||
| 24 h post- immunostimulation |
N-PBS | 4.00 ± 0.00 a | 0.16 ± 0.00 | |
| N-LPS | 2.00 ± 2.83 a | 0.08 ± 0.12 | ||
| N-VA | ND a | - | ||
| H-PBS | 32.00 ± 0.00 b | 0.29 ± 0.00 | ||
| H-LPS | ND a | - | ||
| H-VA | 40.00 ± 5.66 b | 0.33 ± 0.03 | ||
| Chronic hypoxia | After intermittent hypoxia (6 weeks) |
Normoxia | 60.00 ± 11.31 | 0.42 ± 0.05 |
| Hypoxia | 30.00 ± 2.83 | 0.28 ± 0.01 | ||
| 6 days post- immunostimulation |
N-PBS | 54.00 ± 2.83 | 0.39 ± 0.01 | |
| N-LPS | 146.00 ± 8.49 | 0.81 ± 0.04 | ||
| H-PBS | 102.00 ± 36.77 | 0.61 ± 0.17 | ||
| H-LPS | 70.00 ± 8.49 | 0.47 ± 0.04 |
Results expressed as mean ± SD. The letters indicate statistically significant differences between groups (p ≤ 0.05) at the same sampling time. N: normoxia; H: hypoxia; PBS: non-stimulated group; LPS: group inoculated with LPS; VA: group inoculated with the inactivated vaccine. ND: not detected, values ≤ 0.