Table 3.
Peroxidase content of the Chilean meagre mucus samples from acute and chronic hypoxia experiments.
| Experiment | Time Point | Treatment | Peroxidase Activity (U mg−1) | Amount of Peroxidase per 100 µg of Total Protein Extracts (µg) |
|---|---|---|---|---|
| Acute hypoxia | After hypoxia (3 h) | Normoxia | 0.73 ± 0.42 | 0.07 ± 0.03 |
| Hypoxia | 0.97 ± 0.26 | 0.09 ± 0.02 | ||
| After immunostimulation |
N-PBS | 0.20 ± 0.06 a | 0.04 ± 0.00 | |
| N-VA | 0.46 ± 0.01 bc | 0.05 ± 0.00 | ||
| H-PBS | 0.33 ± 0.01 ac | 0.05 ± 0.00 | ||
| H-VA | 0.60 ± 0.01 b | 0.06 ± 0.00 | ||
| 24 h post- immunostimulation |
N-PBS | 0.30 ± 0.05 ab | 0.04 ± 0.00 | |
| N-LPS | 0.85 ± 0.03 c | 0.08 ± 0.00 | ||
| N-VA | 0.29 ± 0.06 ab | 0.04 ± 0.00 | ||
| H-PBS | 0.34 ± 0.02 a | 0.05 ± 0.00 | ||
| H-LPS | 0.14 ± 0.01 bd | 0.03 ± 0.00 | ||
| H-VA | 0.10 ± 0.01 d | 0.03 ± 0.00 | ||
| Chronic hypoxia | After intermittent hypoxia (6 weeks) |
Normoxia | 3.26 ± 0.00 | 0.52 ± 0.00 |
| Hypoxia | 3.32 ± 0.03 | 0.53 ± 0.00 | ||
| 6 days post- immunostimulation |
N-PBS | 0.05 ± 0.01 a | 0.03 ± 0.00 | |
| N-LPS | 2.57 ± 0.01 b | 0.42 ± 0.00 | ||
| H-PBS | 0.65 ± 0.04 c | 0.12 ± 0.01 | ||
| H-LPS | 0.74 ± 0.06 c | 0.13 ± 0.01 |
Results expressed as mean ± SD. The letters indicate statistically significant differences between groups (p ≤ 0.05). N: normoxia; H: hypoxia; PBS: non-stimulated group; LPS: group inoculated with LPS; VA: group inoculated with the inactivated vaccine.