Figure 9.

Treatment of LM81 cells with gemcitabine eliminates CAFs and inhibits the formation of tumor clusters and proliferation of cells. (A) Images of LM1 cells treated with DMSO and with 5 nM of gemcitabine. Cells were plated at low-density (upper) and high-density (bottom). (B) Bar graphs show calculations of high-density plated cells after treatments. ** shows p < 0.01. (C) A western blot was performed with proteins isolated from the 4 experimental plates for each treatment. (D) Summary of the treatments of LM1 cells with gemcitabine. The whole gel images of western blots can be found in File S1.