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Validation of NGS results (left panel) of selected miRNA expression using qPCR (right panel) in the cell lines (A) or EVs (B). MiRNA expression of miR-19a-3p, miR-143-3p and miR-369-3p in cell lines (A) or the expression of miR-143-3p, miR-146a-5p and miR-181a-5p in EVs (B) was analyzed using RT-qPCR and normalized to U6 expression using the 2−ΔΔCT method. Data were analyzed using the Kruskal–Wallis test with Dunn’s post hoc test, and medians ± IQR are shown. * p < 0.05, ** p < 0.01.
