Figure 6.
qPCR analysis of selected miRNA expression in CRC patients‘ samples, in comparison to healthy donors. The expression of seven selected miRNAs in tumor samples obtained from CRC patients (A). miR-182-5p, miR-196-5p, miR-425-5p and miR-429 expression in EVs isolated from patients’ plasma (B). The analysis was performed in CRC patients (n = 9) and healthy donors (n = 9). miRNA expression was analyzed using RT-qPCR and normalized to U6 expression using the 2−ΔΔCT method. Data were analyzed using the Kruskal–Wallis test with Dunn’s post-hoc test (A) or Mann–Whitney test (B), and medians ± IQR are shown. Asterisks mark significant differences: * p < 0.05, ** p < 0.01.