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. Author manuscript; available in PMC: 2024 Jul 12.
Published in final edited form as: Cell Rep. 2024 Jun 11;43(6):114330. doi: 10.1016/j.celrep.2024.114330

Figure 3. Localization of mRNA with normal length CGG repeats is mediated by GQs.

Figure 3.

(A) Schematic illustration of the timing of primary mouse hippocampal neuron transfection and TMPyP4 treatment.

(B) Representative confocal images of MS2-transfected neurons treated with VEH (left) or TMPyP4 (right) at 60× magnification. Scale bar, 20 μm. Green, MS2-reporter; red, Synapsin-mCherry reporter; white, MAP2 (postmitotic neuron marker).

(C and E) Quantification the GFP fluorescence intensity along the length of primary dendrites of GFP+/mCherry+/MAP2+ neurons transfected with 31 CGG (C) or 0 CGG (E) plasmids. Shaded area indicates SEM.

(D and F) Summation of the fluorescence intensity in primary dendrites, normalized for dendritic length in 31 CGG (D) or 0 CGG (F) transfected neurons. Each data point represents single neurons (R1–R3). Data in (C–F) are from N = 3 independent neuronal isolations/biological replicates (18–29 neurons per replicate). Values were normalized to vehicle (VEH) condition for each batch of neurons. (C and E) Two-way ANOVA, significant main effect of treatment ****p < 0.001. (D and F) Welch’s t test; *p < 0.05; ****p < 0.001.