Figure 1.

Alterations of cell kinetics and maturation in the in vitro cachexia model. C2C12 cells were treated with a medium containing cancerous ascites (20% v/v). The medium was changed to ascites-supplemented medium every two days. (A) Cell growth. (B) Apoptotic cells. (C) Number of cells showing cell surface labeling at 144 h. (D) Cell turnover (%) calculated as the unlabeled cell number/total cell number. (E,F) Protein levels of MYH8 and PAX7. * Error bars: standard deviation from three independent trials. Statistical differences were calculated using analysis of variance (ANOVA) with Bonferroni correction. C, control; CX, in vitro cachexia model; MC, medium change; MYH8, myosin heavy chain-8; PAX7, paired box protein-7.