Table 2.
Changes in the levels of aging markers in in vitro cachexia model in C2C12 cells.
| Parameter | 0 | 1 d | 6 d | |||
|---|---|---|---|---|---|---|
| C | CX | C | CX | C | CX | |
| MDA (μg/g) | 8.1 ± 0.04 | 8.1 ± 0.1 | 8.2 ± 0.1 | 9.5 ± 0.4 * | 8.2 ± 0.4 | 13.2 ± 0.5 * |
| 4HNE (ng/g) | 45 ± 0.03 | 46 ± 0.04 | 46 ± 0.6 | 52 ± 1.1 * | 47 ± 1.2 | 64 ± 1.6 * |
| AGE (ng/g) | 82 ± 0.8 | 81 ± 0.5 | 83 ± 1.2 | 87 ± 1.3 * | 89 ± 0.9 | 101 ± 2.0 * |
| HMGB1 (μg/g) | 5.7 ± 0.6 | 6.0 ± 1.0 | 7.3 ± 0.6 | 7.7 ± 0.6 | 5.7 ± 0.6 | 15.3 ± 1.5 * |
| COL (μg/g) | 12.5 ± 0.08 | 12.5 ± 0.08 | 12.8 ± 0.13 | 13.2 ± 0.2 * | 12.6 ± 0.13 | 14.6 ± 0.36 * |
| TV (%) | 99.7 ± 0.6 | 100.0 ± 1.0 | 98.0 ± 1.0 | 95.3 ± 1.2 * | 97.0 ± 1.0 | 89.7 ± 0.6 * |
| βGAL (ng/g) | 8.2 ± 0.08 | 8.1 ± 0.05 | 8.1 ± 0.05 | 8.3 ± 0.12 * | 8.2 ± 0.08 | 8.7 ± 0.13 * |
| SDS-MYL1 (pg/g) | 91 ± 0.5 | 91 ± 00.9 | 91 ± 0.9 | 87 ± 1.0 * | 92 ± 0.5 | 78 ± 1.2 * |
The levels of aging markers were examined in C2C12 mouse myoblasts cultured in a medium containing cancer ascites fluid (20%, v/v). * Statistical differences (C vs. CX) were calculated by ordinal analysis of variance (ANOVA) with Bonferroni correction. MDA, malondialdehyde; 4HNE, 4-hydroxynonenal; AGE, advanced glycation end products; HMGB1, high-mobility group box-1; COL, collagen III; TV, telomere volume; βGAL, beta-galactosidase; SDS-MYL1, sodium dodecyl sulfate-soluble myosin light chain-1.