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. 2024 Jun 22;25(13):6869. doi: 10.3390/ijms25136869

Figure 2.

Figure 2

Figure 2

Confocal laser scanning microscopy of internalization of McCoy cells by the predicted cell-penetrating peptide. Mouse fibroblast cells (A) without incubation with peptide CpRE12; after incubation with (B) NBD-F tag without peptide; (C) NBD-F tagged penetratin; (D) NBD-F tagged control peptide CpHM15 from the organism Hirudo medicinalis, identified by the algorithm as non-penetrating; (E) NBD-F tagged predicted peptide CpRE12 from the jellyfish species Rhopilema esculentum. Hoechst 33342 intranuclear localization regions are blue; Alexa Fluor 594 cell wall localisation regions are red and peptide-NBD-F localization regions are green. (Scale bar: 106 μm).