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. 2024 Jun 27;25(13):7026. doi: 10.3390/ijms25137026

Figure 2.

Figure 2

Expression of osteogenic genes: The expression of RUNX2 (A), BMP2 (B), osteocalcin (C), and ALP (D) was evaluated in hADSCs cultured in the presence of 4d-MCF-7-E.M (4 d: red bars), 7d-MCF-7-E.M (7 d: green bars), or 10d-MCF-7-E.M (10 d: blue bars). The mRNA levels for each gene were normalised to glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) and expressed as the fold change (2−ΔΔCt) of the mRNA levels observed in untreated control hADSCs (Ctrl: grey bars), defined as 1 (mean ± SD; n = 6). Data are expressed as the mean ± SD relative to the control (* p ≤ 0.05), (*** p ≤ 0.001), (**** p ≤ 0.0001).