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. 2024 Jun 27;25(13):7026. doi: 10.3390/ijms25137026

Figure 4.

Figure 4

Effects of MCF-7-E.M in ADSCs during adipogenic and osteogenic differentiation: (A) Lipid and calcium accumulation, after 14 days of treatment, in ADSCs exposed to 4d-MCF-7-E.M (4 d: red bars), 7d-MCF-7-EM (7 d: green bars), or 10d-MCF-7-EM (10 d: blue bars). Positive controls (CTRL+: light grey bars) are ADSCs cultured in adipogenic or osteogenic conditioned medium. Scale bar = 100 μm. The percentage of lipid accumulation (B) or mineralisation (C) was calculated using ImageJ. Data are expressed as the mean ± SD and are representative of 6 different experiments. An average was made from three technical replicates (**** p ≤ 0.0001).