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. 2024 Jun 30;25(13):7242. doi: 10.3390/ijms25137242

Figure 2.

Figure 2

Design of αFAP TMs for redirection of UniCAR T-cells to FAP+ target cells. (A) Schematic representation of the UniCAR system and αFAP TMs targeting the FAP antigen. UniCAR T-cells are second generation CARs composed of an extracellular scFv targeting the peptide epitope E5B9, along with CD28 transmembrane and intracellular signaling domains. The presence of an adaptor target module (TM), i.e., αFAP TM, containing either E5B9 or E5B9L peptide, is required to redirect UniCAR T-cells to FAP+ cells and trigger effector cell functions. (B) Different formats of αFAP TMs were generated: scFv TMs and IgG4-based TMs. Both formats contain either the E5B9 peptide epitope or the E5B9L variant. LP: Igκ leader peptide; VH: variable domain of the antibody heavy chain; VL: variable domain of the antibody light chain; Fc: fragment crystallizing; His: hexa-histidine tag.