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Release of pro-inflammatory cytokines by UniCAR T-cells upon TM cross-linking of effector and target cells. UniCAR T-cells and SCP-1 Luc (A) or HT1080 hFAP Luc (B) were incubated at an E:T ratio of 5:1 in the presence of different αFAP TM concentrations. After 8h, IFNγ, IL-2 and TNFα concentrations in co-culture supernatants were determined by ELISA. Results are shown as mean ± SD for three independent T-cell donors.
