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. 2024 Jun 29;25(13):7180. doi: 10.3390/ijms25137180

Figure 6.

Figure 6

DNA methylation changes for the CHH sequence context are involved in cytokinin and auxin homeostasis in the two types of pear flower buds. (a) Methylation levels (MLs) of differentially expressed genes associated with differentially methylated regions (DMEGs) involved in the synthesis, transport, signal transduction, and catabolism pathways of cytokinin and auxin (in normal (CKM) and wizened (SM) flower buds). (b) Quantitative real−time PCR (qPCR) validation of relative expression levels of the genes described above. (c) Quantification of cytokinin and auxin concentrations in CKM and SM. Data (a–c) are presented as the mean ± standard deviation (SD). Vertical bars represent SD of the mean of at least three biological replicates. SPSS26 was used for statistical analysis. Student’s t-test was used for comparing two samples; asterisks indicate statistically significant differences between normal (CKM) and wizened (SM) flower buds (** means p < 0.01).