Figure 5.

OFI-EVs inhibit LPS-induced inflammatory response in the THP-1 cell line. The effect of OFI-EVs (5 and 20 µg/mL) on the production of IL-6 (A,B), IL-8 (C,D), and TNF-α (E,F) was measured via an ELISA assay (A,C,E) and qRT-PCR (B,D,F). THP-1 cells were pretreated with OFI-EVs for 24 h, then stimulated with LPS (1 µg/mL) for 24 h (ELISA assay) or 4 h (qRT-PCR). Results are expressed as the means of three independent experiments ± S.D (n = 3). ### p < 0.005 vs. CTL, ⁺ p < 0.05, ⁺⁺ p < 0.01, and ⁺⁺⁺ p < 0.005 OFI-EVs vs. LPS.