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. 2024 Jul 12;22:412. doi: 10.1186/s12951-024-02683-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Rescue effect of GSH-CDs on NPCs senescence and mitochondrial dysfunction via the removal of excessive ROS. (a) DHE fluorescence staining images of NPCs in different groups (Scale bar = 200 μm). (b) Semiquantitative analysis of DHE staining. (c) Images of mitochondrial fluorescence staining (Scale bar = 200 μm). (d) Semiquantitative analysis of mitochondrial fluorescence intensity. SA-β-gal staining (e) of NPCs and semiquantitative analysis (f) of the percentage of SA-β-Gal-positive cells in different groups (Scale bar = 200 μm). (g) Immunofluorescence staining results of p21 in different groups (Scale bar = 200 μm). (h) Semiquantitative analysis of the fluorescence intensity of p21. (* indicates a p value < 0.05, ** indicates a p value < 0.01, *** indicates a p value < 0.001, **** indicates a p value < 0.0001)