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. 2024 Jul 12;24:257. doi: 10.1186/s12866-024-03400-7

Table 1.

Primers used in this study

Primer Targets Sequences (5’-3’) Amplification
conditions
Amplification product size (bp) Ref.
94 °C (1 min)
TLR2

F: ATCCTCCAATCAGGCTTCTCT

R: ACACCTCTGTAGGTCACTGTTG

53 °C (30 s)

72 °C (1 min)

163 [36]
94 °C (1 min)
TLR4

F: AAGCCGAAAGGTGATTGTTG

R: CTGAGCAGGGTCTTCTCCAC

53 °C (30 s)

72 °C (1 min)

143 [37]
94 °C (1 min)
GAPDH

F: GAGCCACATCGCTCAGACAC

R: CATGTAGTTGAGGTCAATGAAGG

58 °C (30 s)

72 °C (1 min)

108 [36]