Table 2.
Physiochemical characterization of optimized N/Se@TCsNPs, N/Se@TCsNPs: nisin and selenium encapsulated in chitosan nanoparticles; cs: Chitosan, TCs: Thiolated Chitosan, N@TCsNPs: Nisin encapsulated in thiolated chitosan nanoparticles, and N/Se@CsNPs: Nisin and selenium encapsulated in chitosan nanoparticles
| Formulation | Cs (mg/mL) |
Cysteine (mg/mL) |
Nisin (mg/mL) |
Selenium (µg/mL) |
PH | Z-average size (nm) | PDI | Thiol content µmol/g |
|---|---|---|---|---|---|---|---|---|
| TCs І | 10 | 10 | - | - | 5 | - | - | 484.3 ± 9.01 |
| TCs ІІ | 10 | 15 | - | - | 5 | - | - | 495 ± 5.0 |
| TCs ІІІ | 10 | 20 | - | - | 5 | - | - | 512.66 ± 7.33 |
| N@TCsNPs І | 10 | 20 | 2 | - | 7 | 59.6 ± 0.52 | 0.49 ± 0.05 | 512.66 ± 7.33 |
| N@TCsNPsІІ | 10 | 20 | 2.5 | - | 7 | 87.25 ± 24.92 | 0.25 ± 0.03 | 512.66 ± 7.33 |
| N@TCsNPsІІІ | 10 | 20 | 5 | - | 7 | precipitation | - | - |
| N/Se@TCsNPsІ | 10 | 20 | 2.5 | 200 | 7 | 136.26 ± 43.17 | 0.27 ± 0.07 | 512.66 ± 7.33 |
| N/Se@TCsNPsІІ | 10 | 20 | 2.5 | 250 | 7 | precipitation | - | - |
| N/Se@TCsNPsІІІ | 10 | 20 | 205 | 300 | 7 | Aggregation immediately | - | - |