Table 3.

Minimum inhibitory concentration values (mg/mL) of Se@TCsNPs, N@TCsNPs, N/Se@TCsNPs, N/Se@CsNPs, pure nisin and sodium selenite against microbial strains, N/Se@TCsNPs: Nisin and selenium encapsulated in thiolated chitosan nanoparticles, N/Se@CsNPs: Nisin and selenium encapsulated in chitosan nanoparticles, Se@TCsNPs: selenium encapsulated in thiolated chitosan nanoparticles

Microorganism type	Strain	Se@TCsNPs	N@TCsNPs	N/Se@TCsNPs	N/Se@CsNPs	Nisin	Sodium selenite
gram-positive	S aureus	25 ± 0.05	12.5 ± 0.32	1.5 ± 0.08	1.5 ± 0.4	25 ± 0.01	100 ± 0.04
	L. monocytogenes	50 ± 0.16	12.5 ± 0.48	3.125 ± 0.19	6.25 ± 0.02	25 ± 0.07	200 ± 0.05
gram-negative	E. coli	25 ± 0.42	50 ± 0.75	6.25 ± 0.53	12.5 ± 0.01	200 ± 0.02	˃500
	S. enterica. Sub. enterica	100 ± 0.04	200 ± 0.01	12.5 ± 0.26	12.5 ± 5.03	˃500	˃500
	S.dysenteriae	50 ± 0.05	100 ± 0.35	6.25 ± 0.05	50 ± 4.61	˃500	˃500
	V.cholerae O1 El Tor	25 ± 0.01	100 ± 4.29	12.5 ± 1.01	25 ± 0.04	˃500	˃500
	C. jejuni	50 ± 0.01	200 ± 10.38	25 ± 4.04	25 ± 0.07	˃500	˃500