Fig. 1.

Flow chart. The peripheral blood of 22 septic patients and 10 healthy volunteers was collected for Bulk RNA sequencing and differential expression analysis. At the same time, the TCMSP database was used to obtain the active ingredients and targets of Scutellariae Radix. Disease-drug intersection targets are used for GO and PPI analysis. Core targets were screened using survival analysis and meta-analysis, then single-cell RNA sequencing was performed on core targets to clarify cell line localization. Subsequently, the potential active ingredients and targets of Scutellariae Radix in the treatment of sepsis were screened. Finally, molecular docking experiments were performed to further verify the interrelationship between the drug active ingredient and the target