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Coumestrol affects ferroptosis pathway in CRC cell lines. SLC39A8 expression decreased at both mRNA (A) and protein levels (B) in both COLO 205 and HCT 116 cell lines after 96 hours of siRNA interference. Coumestrol could not upregulate ferroptosis pathway-related genes in CRC cell lines treated with SLC39A8 interference (C-D).
