TABLE 2.
rcAAV contamination in rAAV preparations using various helper plasmidsa
| Helper plasmid | rcAAV contamination at dilution:
|
rcAAV contamination of vectors (large scale, second round) | ||
|---|---|---|---|---|
| 1:10 | 1:100 | 1:1,000 | ||
| pAd/AAV | + | + | − | + |
| pCLR1 | − | − | − | − |
| pCLR1-1.5k | − | − | − | − |
| pCLR1-2.0k | − | − | − | ND |
| pCLR-3.5k | − | − | − | ND |
| pCLR-C3k | − | − | − | ND |
| pCLR1-C3k | − | − | − | − |
| p5E18 | + | − | − | ND |
a
The experiments were carried out as described in Fig. 2. Columns 2 to 4 are based on virus preparations from ∼2 × 107 cells. The viruses were diluted as indicated. The last column shows the vectors prepared from 2 × 108 cells. The vectors were not diluted and were subjected to two rounds of amplification for rcAAVs. ND, not determined. See Materials and Methods for more details on the rcAAV determinations.