Zebrafish taste buds, transversal view. The immunohistochemical detection (using a peroxidase method) of Piezo 1. (a) In the cutaneous taste buds, the Merkel-like cells (arrowheads), light cells (blue arrows), and dark cells (red arrows) showed immunopositivity to Piezo 1. (b) In the pharyngeal taste buds, the Merkel-like cells (arrowheads), light cells (blue arrows), dark cells (red arrows), and supporting cells (black arrows) showed immunoreactivity to Piezo 1. (c) In the lingual taste buds, the light cells (blue arrow) and supporting cells (black arrows) were Piezo 1-immunolabelled. Magnification, 40×; scale bar, 20 µm.