Figure 6.

Zebrafish taste buds, transversal view. (a,b) The immunohistochemical detection (using a peroxidase method, Haematoxylin-stained) of Piezo 2. The light cells (blue arrows), dark cells (red arrows), and nerve cells (black arrows) were Piezo 2-immunopositive. (a) Isolated chemosensory cells (black arrowhead) were immunoreactive to Piezo 2. (c–e) The multiple focal planes of the Piezo 2 immunohistochemical detections (using a fluorescence method). The light cells (white arrows) and dark cells (yellow arrows) were immunoreactive to Piezo 2. Magnification, 40×; scale bar, 20 µm.