Table 4.
Primary Antibodies |
Supplier | Catalogue Number | Source | Dilution | Antibody ID |
---|---|---|---|---|---|
Piezo 1 | Invitrogen-Thermo Fisher Scientific, Waltham, MA USA |
PA5-106296 | rabbit | 1:100 | AB_2853973 |
Piezo 2 | Invitrogen-Thermo Fisher Scientific, Waltham, MA USA |
PA5-72975 | rabbit | 1:100 | AB_2718829 |
Calretinin | Santa Cruz Biotechnology, Inc., Dallas, Texas, USA | sc-11644 | goat | 1:100 | AB_634545 |
Secondary Antibodies |
Supplier | Catalogue Number | Source | Dilution | Antibody ID |
anti-Goat IgG (H + L) Alexa Fluor 594 | Invitrogen-Thermo Fisher Scientific, Waltham, MA USA | A-11058 | donkey | 1:300 | AB_2534102 |
anti-Rabbit IgG (H + L) Alexa Fluor 488 | Invitrogen-Thermo Fisher Scientific, Waltham, MA USA | A-11008 | goat | 1:300 | AB_143165 |
anti-rabbit IgG-peroxidase conjugate | Amersham bioscences, Piscataway, NJ, USA | NA934 | donkey | 1:100 | AB_772206 |