Figure 5.

Time-dependent actin disruption with Cyto-B reduces the size of Cx43 and Cx26 GJCs plaque. HeLa cells transfected with Cx43 or Cx26 ((a) bottom panels) were exposed to Cyto-B for 10, 30, and 60 min. The effect of the drug treatment on plaque formation at the cell-cell borders (yellow arrowheads) was quantified on cells expressing Cx43 (b) or Cx26 (c). Data presented as mean ± SEM (n = 3; 15 cell pairs per condition in cells expressing Cx43; n = 3; 20 cell pairs per condition in cells expressing Cx26); * p < 0.05. Scale bar: 7.2 µm.