Figure 11.

Cyto-B increases the ionic current through Cx43 HCs. Representation of voltage steps protocol used to evaluate total currents in HeLa-Cx43 or HeLa (HP) cell lines in whole-cell configuration. Cells were held at 0 mV and voltage steps (+30 mV increment, 6 s duration) were applied to promote HCs currents, followed by a −40 mV voltage step of 4 s length (a). Representative traces of Cx43 HCs or HeLa Parental currents in untreated control condition; 10 min in the presence of Cyto-B (4 µM) and La³⁺ (200 µM) (b,c). Graphs show the normalized amplitude current of HeLa-Cx43 (d) and HeLa (HP) (e). All treatments were compared to the control condition. Data are presented as mean ± SEM (n = 3); (* p < 0.05).