Figure 9.

Categorization of Dendritic Morphology in Doublecortin (Dcx) Labelled Neurons. (A) Representative phenotypes of Dcx-positive cells ranging from immature, intermediate, mature. (B) Schematic of granule cell layer (GCL) with software generated counting frames (not to scale) for selection of cells for analysis. The profile of Dcx-positive cells falling within counting frame were categorized as one of the phenotypes depicted in A. The GCL of both hemispheres in 5 sections of each of 4 animals per treatment condition were analyzed resulting in assessment of ~200-300 cells/animal (see text). (C) The mean (+/− SEM) proportion of total number of cell analyzed falling within each category in the different treatment conditions reflect a shift in the distribution to fewer Dcx-positive neurons with mature dendritic morphology in developmentally hypothyroid groups. Significant effects in ANOVA for Cell Type and Treatment X Cell Type interaction were followed by Duncan’s Multiple Range Test for between group comparisons. Within each category, * denote significant differences from control (Con-Con), # different from Con-PTU, p<0.05. Calibration bar 10 μM.