Table 1.
Mitochondrial protein coding genes annotated in the Ludwigia grandiflora subsp. hexapetala (Lgh) and Ludwigia peploides subsp. montevidensis (Lpm) mitogenomes. Numbers within parentheses indicate the number of exons for spliced genes. Trans-spliced genes are marked by “*”. Locally collinear blocks (LCBs) correspond to conserved regions between Lgh and Lpm mitogenomes, identified by ProgressiveMauve. The molecular weight and isoelectric point of protein were calculated using the Sequence Manipulation Suite. Hypothetical localization was estimated by Plant-mPLoc.
| Gene | LCB | Molecule | Size (in bp) |
Molecular Weight (in kDa) |
Protein Isoelectric Point | Hypothetical Localization |
|---|---|---|---|---|---|---|
| Complex I (NADH dehydrogenase) | ||||||
| nad1 (5*) | LCB0 | M2 | 1008 | 36.81 | 10.10 | Mitochondrion |
| LCB1 | M1 | |||||
| LCB8 | M1 | |||||
| nad2 (5*) | LCB0 | M2 | 1518 | 55.34 | 9.28 | Mitochondrion |
| nad3 | LCB3 | M1 | 357 | 13.39 | 4.33 | Mitochondrion |
| nad4 (4) | LCB5 | M1 | 1473 | 54.27 | 8.97 | Mitochondrion |
| nad4L | LCB5 | M1 | 303 | 10.94 | 6.70 | Mitochondrion |
| nad5 (5*) | LCB1 | M1 | 1794 | 74.55 | 7.90 | Mitochondrion |
| LCB5 | M1 | |||||
| nad6 | LCB1 | M1 | 618 | 23.45 | 10.53 | Mitochondrion |
| nad7 (5) | LCB8 | M1 | 1182 | 44.32 | 7.87 | Mitochondrion |
| nad9 | LCB5 | M1 | 573 | 22.63 | 8.20 | Mitochondrion |
| Complex II (succinate dehydrogenase) | ||||||
| sdh4 | LCB7 | M1 | 393 | 15.25 | 10.76 | Mitochondrion |
| Complex III (cytochrome c reductase) | ||||||
| cob | LCB5 | M1 | 1182 | 44.04 | 7.52 | Cell membrane |
| Complex IV (cytochrome c oxidase) | ||||||
| cox1 | LCB0 | M2 | 1623 | 58.75 | 9.25 | Mitochondrion |
| cox2 (2) | LCB7 | M1 | 780 | 29.09 | 5.53 | Mitochondrion |
| cox3 | LCB7 | M1 | 798 | 29.54 | 7.53 | Mitochondrion |
| Complex V (ATP synthase) | ||||||
| atp1 | LCB3 | M1 | 1533 | 55.51 | 6.21 | Mitochondrion |
| atp4 | LCB0 | M2 | 573 | 21.25 | 10.26 | Mitochondrion |
| atp6 | LCB8 | M1 | 1044 | 38.30 | 4.80 | Mitochondrion |
| atp8 | LCB7 | M1 | 480 | 18.19 | 9.50 | Mitochondrion |
| atp9 | LCB0 | M2 | 330 | 11.61 | 9.09 | Mitochondrion |
| Cytochrome c biogenesis | ||||||
| ccmB | LCB4 | M1 | 621 | 23.07 | 8.62 | Mitochondrion |
| ccmC | LCB6 | M1 | 723 | 26.92 | 10.98 | Mitochondrion |
| ccmFc (2) | LCB4 | M1 | 1500 | 56.40 | 10.74 | Mitochondrion |
| ccmFn | LCB7 | M1 | 1983 | 74.21 | 10.45 | Mitochondrion |
| Maturase | ||||||
| matR | LCB8 | M1 | 1983 | 73.76 | 10.71 | Chloroplast |
| Protein translocase | ||||||
| mttb | LCB1 | M1 | 768 | 29.07 | 7.75 | Mitochondrion |
| Ribosomal large subunit | ||||||
| rpl2 (3) | LCB0 | M2 | 1389 | 50.69 | 11.28 | Mitochondrion |
| rpl5 | LCB0 | M2 | 564 | 21.55 | 5.89 | Mitochondrion |
| rpl10 | LCB4 | M1 | 486 | 18.53 | 7.45 | Cell membrane, chloroplast, and nucleus |
| rpl16 | LCB0 | M2 | 435 | 15.98 | 11.81 | Mitochondrion |
| Ribosomal small subunit | ||||||
| rps1 | LCB0 | M2 | 693 | 25.87 | 11.89 | Mitochondrion |
| rps3 (2) | LCB0 | M2 | 1659 | 63.57 | 10.96 | Mitochondrion |
| rps4 | LCB0 | M2 | 1047 | 41.18 | 11.63 | Mitochondrion |
| rps7 | LCB5 | M1 | 447 | 17.04 | 11.21 | Chloroplast and mitochondrion |
| rps10 (2) | LCB7 | M1 | 408 | 16.40 | 11.25 | Mitochondrion |
| rps12 | LCB3 | M1 | 378 | 14.30 | 11.80 | Chloroplast and mitochondrion |
| rps13 | LCB0 | M2 | 351 | 13.27 | 11.21 | Mitochondrion |
| rps14 | LCB0 | M2 | 303 | 11.96 | 12.04 | Mitochondrion |
| rps19 | LCB0 | M2 | 285 | 11.20 | 12.32 | Chloroplast |
| LCB7 | M1 | |||||