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. 2024 Jul 3;25(13):7316. doi: 10.3390/ijms25137316

Figure 5.

Figure 5

PPV-6 downregulates expression of the cell cycle/apoptosis markers as well as BrdU incorporation induced by Aβ1-42. (A–D) Primary cortical cultures were exposed to Aβ1-42 (5 μM), PPV-6 (250 μg/mL), or both for 48 h before detection of cyclin D1 (A), PCNA (B), p-Histone H3 (C), as well as pro- and cleaved caspase-3 (D). (E) The cultures were stained with antibodies against BrdU (green) and NeuN (red); Hoechst 33258 (blue) served as counterstaining. White arrows denote mature neurons with BrdU incorporation. Scale bar = 10 μm. Mean ± SEM from N = 3. Data were analyzed by means of a one-way ANOVA followed by a post-hoc Tukey test. *, #, and + all denote p < 0.05.