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. 2000 Dec;74(24):11490–11494. doi: 10.1128/jvi.74.24.11490-11494.2000

FIG. 3.

FIG. 3

FIG. 3

Wild-type and mutant TVB proteins are expressed in transfected human 293 cells. (A) Human 293 cells were transfected with plasmids encoding wild-type or mutant TVB receptors or, for control purposes, with pBK vector (Stratagene). Protein lysates from these cells were subjected to immunoblotting using SUB-rIgG and an HRP-coupled secondary antibody as binding probes. The positions of the full-length and truncated (ΔDD) receptors are indicated. (B) Human 293 cells expressing wild-type and mutant TVB receptors were incubated with SUB-rIgG (TVBS1-based and TVBS3-based constructs) or with SUE-rIgG (TVBT-based constructs) and then with a fluoresceinated secondary antibody (1, 26). The cells were then analyzed by flow cytometry. Open histograms represent data from cells that were not transfected, and the shaded histograms represent data from cells expressing the different TVB proteins as indicated.