FIG. 1.

Graphical representation of promoter fragments used in this study. Shown is the HSV-1 gC promoter from position −35 to +70 bp relative to the transcription start site (gC wt). Black box, gC TATA box (TATAAATT); hatched box, gC initiator element (CCCTCACTACC). Two PstI sites (p) used to elute protein complexes bound to gC promoter fragments are shown. TATA box-deleted (gC Δ TA) and INR-mutated (gC Δ INR) versions of gC-wt are also shown, as is the adenovirus E4 promoter [(G)5E4T] with five 5′ DNA binding sites for the yeast GAL4 transcription factor (gray boxes) and with its TATA box (black box; TATATATA) as described previously (34). Black circles, added biotin moieties.