FIG. 6.

Effect of the gC TATA box on ICP4-mediated recruitment of HA-TFIID to immobilized gC promoter templates. HA-TFIID (4 μl) was incubated with 100 fmol of immobilized wt or TATA box-mutated (ΔTA) gC promoter in the absence or presence of purified ICP4 (300 fmol) and in the absence or presence of poly(dG-dC) (30 ng/μl) in a total volume of 150 μl. Poly(dG-dC) was added either at time zero (lane 3) or following 30 min of incubation at 30°C (lanes 8 to 11). After incubation at 30°C for 30 min (lanes 1 to 3) or for 90 min (lanes 4 to 11), nucleoprotein complexes bound to immobilized promoters were isolated and analyzed as described in Materials and Methods.