Figure 5.

(A) Representation of the Cryo-EM structure Kv7.2 in complex with ztz240 (PDB ID: 7CR1). Kv7.2 is represented by cartoons and surface, while ztz240 is represented by green spheres inside the VSD binding site. Cartoons are colored as follows: PD of one subunit is colored in blue, while the VSD of the same subunit is colored in yellow. The other subunits are represented in gray. (B) Experimental bound conformation of ztz240 (green sticks). Residues of the pocket are represented by yellow sticks, and include Phe137, Asp172, and two gating charge residues Arg207 and Arg210 in the side pocket comprised between S3 and S4 helices (VSD). Hydrogen bonding (yellow dashed line) is found between the ligand and the side chain of Arg210 [11].