FIG. 3.

The terminal repeats in pGET015 are fully functional. Ad5dl312-infected 293 cells were processed at the indicated times time after transfection with psub201(−) or pGET201(+), and equal amounts of the cell pellet (lanes C) or hypotonic lysate (lanes L) were analyzed by Southern blotting using a probe corresponding to the AAV2 rep and cap genes. Markers (lanes M) were prepared by loading a 4.7-kb PvuII fragment from psub201(−) and either left untreated (u) or denatured (d). dsDNA, double-stranded DNA; ssDNA, single-stranded DNA.