Biochemical characteristics of TNBC-derived sera in diet-induced obese and orthotopic mammary fat pad models. (A) Glucose, (B) triglyceride, (C) free fatty acid, (D) total cholesterol, (E) HDL cholesterol, (F) LDL/VLDL cholesterol levels in TNBC-derived sera of ND, ND-IF, HFD, and HFD-IF mice (n = 6/each group) using quantitative colorimetric assays with duplicate measurements. Box plots were graphed from MS Excel. * p < 0.05 between groups as analyzed by ANOVA and Tukey’s pairwise comparison tests. IF: intermittent fasting of 24 h cycle; HDL: high-density lipoprotein; LDL: low-density lipoprotein; VLDL: very-low-density lipoprotein.