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. 2024 Jul 1;16(13):2101. doi: 10.3390/nu16132101

Figure 8.

Figure 8

Immunohistochemical evaluation of immune cells in tumor tissues from diet-induced obese and orthotopic mammary fat pad models. Histological features of (A) F4/80 and (B) LY-6G-positive cells in tumor tissues from ND, ND-IF, HFD, and HFD-IF mice. (C) Comparative effects of HFD and HFD-IF on F4/80 and LY-6G expression levels in tumor tissues. Tumor lysates were prepared, and Western blots were carried out using antibodies specific to F4/80 and LY-6G. β-actin was used as a loading control. All data are shown as mean ± SD. * p < 0.05 in each group as calculated by the paired Student’s t-test. Histological features of (D) arginase- and (E) CD11c-positive cells in tumor tissues from ND, ND-IF, HFD, and HFD-IF mice. IF: intermittent fasting of 24 h cycle; tm: tumor tissue; ft: fat tissue; nc: necrotic region.