TABLE 5.
Protection of mice from shedding of rotavirus antigen after i.n. immunization with overlapping fragments of the CD region of VP6 and LT(R192G)a
| Immunogen | Avg quantity (ng) ± SEM of rotavirus antigen shed/mouse/day | % Reduction in shedding relative to control group |
|---|---|---|
| None | 207 ± 57 | |
| CD | 9 ± 4b | 96 |
| CD1 | 24 ± 7bc | 88 |
| CD2 | 32 ± 7bd | 84 |
| CD3 | 167 ± 51 | 19 |
| CD4 | 17 ± 5b | 92 |
a
Groups of eight BALB/c mice were immunized i.n. with two 9-μg doses of chimeric CD, CD1, CD2, CD3, or CD4 protein and 10 μg of LT(R192G). Immunized mice were challenged with EDIM 4 weeks after the last immunization. Shedding of rotavirus antigen (nanograms per mouse per day) was determined by ELISA during the subsequent 7 days.
b
Significantly (P ≤ 0.004) less than that of the mock-immunized group.
c
Not significantly (P = 0.053) more than that of the CD4-immunized group.
d
Not significantly (P = 0.057) more than that of the CD4-immunized group.